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Table 1: RNAi Vectors

The RNAi vectors have been deposited at ABRC; they are not available through the ChromDB database.

TO ORDER PLASMIDS: The ABRC stock number is a hot link that will bring up the TAIR order page for each vector. You will be directed to the TAIR website and the TAIR vector page for the plasmid of interest. At the bottom left side of this new page, there is a button "Order from ABRC". Click on this button which brings up a login page. If you are a registered community user at TAIR, type in your information and proceed to the ordering instructions. If you are not registered at TAIR, click on the "Register as a new TAIR user" (this link is within the sentence "If your personal profile does not exist in our database, Register as a new TAIR user). This will enable you to register as a new TAIR user and order the plasmid.

Each plasmid name is a hot link that brings up a restriction enzyme map of the plasmid, as well as a text file of the sequence and a Gene Construction Kit file (http://www.textco.com).

A. Plasmids utilizing inner and outer restriction enzymes sites with the two-step ligation strategy.

Plasmid	ABRC Stock Number	Bacterial Selectiona	Plant Selection	Promoter Size (bp)b	Poly A Signal	Stuffer Fragment
pFGC1008c	CD3-446	Cm	hygromycin	1400	ocs3'	Gus
pFGC5941d	CD3-447	Km	basta	1400	ocs3'	ChsA intron
pGSA1131	CD3-449	Cm	basta	200	ocs3'	Gus
pGSA1165	CD3-450	Cm	kanamycin	200	ocs3'	Gus
pGSA1204	CD3-451	Cm	basta	500	ocs3'	Gus
pGSA1276	CD3-453	Cm	kanamycin	500	ocs3'	Gus
pGSA1252	CD3-452	Cm	basta	200 (3x)	ocs3'	Gus
pGSA1285	CD3-454	Cm	kanamycin	200 (3x)	ocs3'	Gus
pMCG161	CD3-459	Cm	basta	1400	ocs3’	Rice waxy intron'

B. Arabidopsis plasmids with unique AscI restriction sites enabling transfer of inverted repeats

Plasmid	ABRC Stock Number	Bacterial Selection	Plant Selection	Promoter	Poly A Signal
pGSA1403	CD3-455	Cm	kanamycin	200 (3x)	ocs3'
pGSA1427	CD3-456	Cm	basta	200 (3x)	ocs3'
pGSA1561	CD3-457	Cm	basta	200 (3x)	nos3'
pGSA1783	CD3-458	Cm	basta	200 (3x)	ssu3'

^aCm: chloramphenicol; Km: kanamycin

^bA longer CaMV 35S promoter may drive dsRNA production in more tissue types.

^cGenBank accession AY310333

^dGenBank accession AY310901

Acknowledgements: ChromDB is funded by a grant from the National Science Foundation Plant Genome Research Project(#DBI-0421679). ChromDB server is hosted by the Biotechnology Computing Facility at the University of Arizona.