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Table 1: RNAi Vectors

The RNAi vectors have been deposited at ABRC; they are not available through the ChromDB database.

TO ORDER PLASMIDS: The ABRC stock number is a hot link that will bring up the TAIR order page for each vector. You will be directed to the TAIR website and the TAIR vector page for the plasmid of interest. At the bottom left side of this new page, there is a button "Order from ABRC". Click on this button which brings up a login page. If you are a registered community user at TAIR, type in your information and proceed to the ordering instructions. If you are not registered at TAIR, click on the "Register as a new TAIR user" (this link is within the sentence "If your personal profile does not exist in our database, Register as a new TAIR user). This will enable you to register as a new TAIR user and order the plasmid.

Each plasmid name is a hot link that brings up a restriction enzyme map of the plasmid, as well as a text file of the sequence and a Gene Construction Kit file (http://www.textco.com).

A. Plasmids utilizing inner and outer restriction enzymes sites with the two-step ligation strategy.

Plasmid

ABRC Stock Number

Bacterial Selectiona

Plant Selection

Promoter Size (bp)b

Poly A Signal

Stuffer Fragment

pFGC1008c

CD3-446

Cm

hygromycin

1400

ocs3'

Gus

pFGC5941d

CD3-447

Km

basta

1400

ocs3'

ChsA intron

pGSA1131

CD3-449

Cm

basta

200

ocs3'

Gus

pGSA1165

CD3-450

Cm

kanamycin

200

ocs3'

Gus

pGSA1204

CD3-451

Cm

basta

500

ocs3'

Gus

pGSA1276

CD3-453

Cm

kanamycin

500
ocs3'

Gus

pGSA1252

CD3-452

Cm

basta

200 (3x)

ocs3'

Gus

pGSA1285

CD3-454

Cm

kanamycin

200 (3x)

ocs3'

Gus

pMCG161

CD3-459

Cm

basta

1400

ocs3’

Rice waxy intron'


B. Arabidopsis plasmids with unique AscI restriction sites enabling transfer of inverted repeats

Plasmid

ABRC Stock Number

Bacterial Selection

Plant Selection

Promoter

Poly A Signal

pGSA1403

CD3-455

Cm

kanamycin

200 (3x)

ocs3'

pGSA1427

CD3-456

Cm

basta

200 (3x)

ocs3'

pGSA1561

CD3-457

Cm

basta

200 (3x)

nos3'

pGSA1783

CD3-458

Cm

basta

200 (3x)

ssu3'

aCm: chloramphenicol; Km: kanamycin

bA longer CaMV 35S promoter may drive dsRNA production in more tissue types.

cGenBank accession AY310333

dGenBank accession AY310901